NAD+ (Nicotinamide Adenine Dinucleotide)
A critical molecule every cell needs to produce energy and repair DNA. Your levels drop by 50% by age 50 and 80% by age 80. It activates your longevity genes (sirtuins), powers your DNA repair crew (PARP enzymes), and is essential for your mitochondria to make energy. Injecting it bypasses the absorption problems you get with oral supplements. When combined with SS-31, it's considered one of the most powerful peptide combos available.
What to Expect
Week 1–2
Increased energy and mental clarity, often noticeable within hours of IV infusion. Improved exercise recovery. Possible flu-like symptoms during infusion.
Week 3–6
Sustained energy improvements. Better mitochondrial function reflected in endurance and recovery. Improved sleep quality. Skin appearance may improve.
Week 8+
Cumulative cellular repair benefits. Improved metabolic biomarkers. Sustained cognitive clarity. DNA repair capacity enhanced. Anti-aging effects building.
Common Side Effects
Scientific Overview
Nicotinamide adenine dinucleotide (NAD+) is a critical coenzyme in redox reactions and a substrate for NAD+-consuming enzymes including sirtuins (SIRT1-7), PARPs, and CD38. Intracellular NAD+ levels decline approximately 50% between ages 40–60, correlating with mitochondrial dysfunction and age-related metabolic decline. Exogenous NAD+ supplementation (IV or subcutaneous) bypasses the rate-limiting step of biosynthesis (NAMPT) and has been shown to improve mitochondrial membrane potential, cellular energy production, and DNA repair capacity in preclinical models.
Dosing
25-50 mg subq, 3 times a week on an empty stomach. Start at 25 mg. Can be mixed in the same syringe with SS-31. Flushing (warm, red skin) is common if you go above 100 mg.
Practical Guide
Reconstitution
IV: administered by clinic — no self-reconstitution. SubQ: mix 100-500mg vial with 1-2mL sterile water per pharmacy instructions.
Storage
Refrigerate reconstituted solution. Use within 7-14 days. Very sensitive to heat and light.
Injection Sites
SubQ injections may sting/burn — inject slowly. IV infusions take 2-4 hours to minimize flushing. Start with lower doses.
Timing
Morning or early afternoon to avoid sleep interference from energy boost. IV sessions best scheduled with 3-4 hours available.
Food
IV: light meal beforehand to reduce nausea. SubQ: no specific food interactions.
Benefit Profile
Medical Considerations
Contraindications
- ✕Active cancer (NAD+ fuels rapid cell proliferation)
- ✕Pregnancy/nursing
- ✕Bipolar disorder (can trigger mania in susceptible individuals)
Drug Interactions
Recommended Monitoring
- →Blood pressure during IV infusion
- →Liver function tests if using high doses
- →Monitor for mania symptoms if psychiatric history
This information is for educational purposes only. Always consult a qualified healthcare provider before starting any peptide protocol.
Published Research
NAD(+) precursor supplementation in human ageing: clinical evidence and challenges.
NAD(+) Boosting Strategies.
Regulation of and challenges in targeting NAD(+) metabolism.
Roles of NAD(+) in Health and Aging.
The therapeutic perspective of NAD(+) precursors in age-related diseases.
NAD metabolism and heart failure: Mechanisms and therapeutic potentials.
Targeting NAD(+) metabolism: dual roles in cancer treatment.
Defining NAD(P)(H) Catabolism.
NAD(+)-Consuming Enzymes in Stem Cell Homeostasis.
NAD(+) metabolism in peripheral neuropathic pain.